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Cloning and Expression
Transfection Kits and Reagents
Transfection Reagents
ViaFect Transfection Reagent
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ViaFect Transfection Reagent
ViaFect Transfection Reagent
ViaFect Transfection Reagent from Promega
(2)
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Citations:
(134)
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ViaFect Transfection Reagent
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Description
ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ViaFect Transfection Reagent
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E4981
Quantity
0.75ml
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (134)
(21)
Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining
STAR protocols
May 12, 2023
Rimmer N, Liang CY, Coelho R, Lopez MN, Jacob F.
Biolabs,https://nebasechanger.neb.com/ 4Peaks,Nucleobytes,https://nucleobytes.com/4peaks/ ,, Other,Other,Other ,,
ViaFect™ Transfection Reagent
,
Promega
,Cat#
E4981
Precision Plus Protein Dual Color Standards
,
Bio-Rad
,Cat#
1610374
T4 DNA Ligase
,
Promega
,Cat# M180B 2×
(22)
Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1
Journal of Chemical Information and Modeling
April 10, 2023
Alessandro Nicoli, Franziska Haag, Patrick Marcinek, Ruiming He, Johanna Kreißl, Jörg Stein, Alessandro Marchetto, Andreas Dunkel, Thomas Hofmann, Dietmar Krautwurst,
et al.
Germany) at 12,000 cells/well overnight. Then, cells were
transfected
utilizing 0.75 μL/well ViaFect (#
E4981
,
Promega
, USA) with the following constructs: 100 ng/well of the respective OR construct, 50 ng/well
(23)
COP I and II dependent trafficking controls ER-associated degradation in mammalian cells
iScience
March 17, 2023
Navit Ogen-Shtern, Chieh Chang, Haddas Saad, Niv Mazkereth, Chaitanya Patel, Marina Shenkman, Gerardo Z. Lederkremer
A,
Sigma
,Cat#
B6542
cOmplete™ Protease Inhibitor Cocktail,Roche,Cat#11697498001
ViaFect™ Transfection Reagent
,
Promega
,Cat#
E4981
DMEM,
Gibco
,Cat# 41965-039
Trypsin
,"Biological Ind., Israel",Cat# 04-007- 1A ,,
Experimental models
:
(24)
A Small Molecule That In Vitro Neutralizes Infection of SARS-CoV-2 and Its Most Infectious Variants, Delta, and Omicron
Biomedicines
March 15, 2023
Reyes-Alcaraz A, Qasim H, Merlinsky E, Fox G, Islam T, Medina B, Schwartz RJ, Craft JW Jr, McConnell BK.
production. We
transfected
the cells using Viafect (
Promega Corporation
, Madison, WI, USA, Cat. No.
E4982
) following the manufacturer’s instructions and using the following plasmid mix per well of a six-well
(25)
A High-Throughput Strategy for T-Cell Receptor Cloning and Expression
Methods in Molecular Biology
March 6, 2023
Qiong Xia, Huang Huang, Mark M. Davis
QIAprep Spin Miniprep Kit (
Qiagen
, Cat. no.
27106
)
ViaFect™ Transfection Reagent
(
Promega
, Cat. no.
E4981
). Lenti-X™ Concentrator (TaKaRa, Cat. no. 631231). HEPES (Gibco, Cat. no. 15630-080) Polybrene 2.4
(26)
The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathway
Acta Pharmaceutica Sinica B
March 1, 2023
Minxuan Xu, Jun Tan, Liancai Zhu, Chenxu Ge, Wei Dong, Xianling Dai, Qin Kuang, Shaoyu Zhong, Lili Lai, Chao Yi,
et al.
Watertown, MA, USA). Vectors were
transfected
into L02 cells via
ViaFect™ Transfection Reagent
(Cat.:
E4982
,
Promega
, Beijing, China) according to the product specification. Moreover, to further study the biological
(27)
LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis
Cell death & disease
February 27, 2023
Ye D, Liu H, Zhao G, Chen A, Jiang Y, Hu Y, Liu D, Xie N, Liang W, Chen X,
et al
.
HY-17559, MedChemExpress).
Lentivirus
packaging and
infection
pMD2.G/psPAX2 plasmids and ViaFect (
E4981
,
Promega
)
transfection reagent
were used in the
lentivirus
packaging system. HEK-
293T cell
supernatants
(28)
Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling
Viruses
February 25, 2023
Evans JG, Novotny LA, Meissner EG.
penicillin/streptomycin, according to ATCC guidelines. Wild-type HEK293T cells were
transfected
using ViaFect (
E4981
,
Promega
, Madison, WI, USA) with empty vector (pTRE-Tight-IP) or pTRE-Tight-IP vectors expressing FLAG-Isoforms
(29)
TFEB-mediated lysosomal exocytosis alleviates high-fat diet-induced lipotoxicity in the kidney
JCI insight
February 22, 2023
Nakamura J, Yamamoto T, Takabatake Y, Namba-Hamano T, Minami S, Takahashi A, Matsuda J, Sakai S, Yonishi H, Maeda S,
et al
.
inserted into vector px458, and the gRNA constructs were
transfected
into PTECs using ViaFect (
Promega
,
E4981
)
transfection reagent
. GFP-positive single cells were sorted by FACS into 96-well plates. Candidate
(30)
ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy
Journal of cell science
February 1, 2023
Sánchez-Martín P, Kriegenburg F, Alves L, Adam J, Elsaesser J, Babic R, Mancilla H, Licheva M, Tascher G, Münch C,
et al
.
5′-AAGUUCCUGCGAGAAAUGGAUUU-3′;
YKT6
siRNA2: 5′-AAGUGAGAUCUCCGCUUGCAGUU-3′). siRNAs were
transfected
using ViaFect (
E4981
,
Promega
) according to the manufacturer's instructions. When indicated, cells were treated with 200
(31)
Repeat-associated non-AUG translation induces cytoplasmic aggregation of CAG repeat-containing RNAs
Proceedings of the National Academy of Sciences
January 17, 2023
Michael R. Das, Yeonji Chang, Rachel Anderson, Reuben A. Saunders, Nan Zhang, Colson P. Tomberlin, Ronald D. Vale, Ankur Jain
plasmid was mixed with Opti-MEM reduced serum media and 1.5 µL
Viafect transfection reagent
(
Promega
,
E4981
) for a total volume of 50 µL. 10 µL of this mixture was added per well of U-2OS cells grown in a 96-well
(32)
EWSR1 prevents the induction of aneuploidy through direct regulation of Aurora B
Frontiers in cell and developmental biology
January 1, 2023
Kim H, Park H, Schulz ET, Azuma Y, Azuma M.
were
transfected
with the mAID-donor and guide RNA (gRNA) plasmid constructs using ViaFect (
Promega
, #
E4981
) (Russell et al., 2010; Mali et al., 2013; Hassebroek et al., 2020). The
transfected
cells were cultured
(33)
Andrographolide ameliorates hepatic steatosis by suppressing FATP2-mediated fatty acid uptake in mice with nonalcoholic fatty liver disease
Journal of natural medicines
January 1, 2023
Ran LS, Wu YZ, Gan YW, Wang HL, Wu LJ, Zheng CM, Ming Y, Xiong R, Li YL, Lei SH,
et al
.
CW2106S, China) and
transfected
into LO2 cells with the
ViaFect™ Transfection Reagent
(
Promega
, cat#
E4981
, USA). Fatty acid uptake
assay
The fatty acid uptake ability of LO2 cells was determined by the
Screen Quest™ Fluorimetric Fatty Acid Uptake Assay Kit
(34)
Assessment of protein inclusions in cultured cells using automated image analysis
STAR Protocols
December 16, 2022
Luke McAlary, Victoria K. Shephard, Mine Sher, Lauren J. Rice, Justin J. Yerbury, Neil R. Cashman, Steven S. Plotkin
assays
,, TransIT-X2
Transfection Reagent
,Mirus Bioscience,Cat# MIR 6000
ViaFect Transfection Reagent
,
Promega
,Cat#
E4981
GeneJET Endo-Free Plasmid Maxiprep Kit
,
Thermo Fisher Scientific
,Cat#
K0861
GeneJET Plasmid Miniprep Kit
(35)
LOTUS suppresses amyloid β-induced dendritic spine elimination through the blockade of amyloid β binding to PirB
Molecular medicine (Cambridge, Mass.)
December 12, 2022
Kawaguchi Y, Matsubayashi J, Kawakami Y, Nishida R, Kurihara Y, Takei K.
encoding EGFP was
transfected
into cultured hippocampal
neurons
(5.0 × 104 cells/well) with Viafect (
E4981
,
Promega
) on DIV11for visualizing
dendritic spine
morphology in primary hippocampal
neurons
to assess
(36)
Fusion of the β(2)-adrenergic receptor with either Gαs or βarrestin-2 produces constitutive signaling by each pathway and induces gain-of-function in BEAS-2B cells
FASEB bioAdvances
December 1, 2022
Lucero-Garcia Rojas EY, Reyes-Alcaraz A, Ruan K, McConnell BK, Bond RA.
βarr2‐LgBiT or Gαs‐LgBiT. We prepared and added 0.3 μl of
ViaFect™ Transfection Reagent
(
Promega
Cat. No.
E4982
) to each well. First, we tested four receptor/βarr2 plasmid combinations to the plasmid combination
(37)
Phosphorylated NFS1 weakens oxaliplatin-based chemosensitivity of colorectal cancer by preventing PANoptosis
Signal Transduction and Targeted Therapy
December 1, 2022
Jin-Fei Lin et al.
Ltd. (Suzhou, China). The plasmids were
transfected
into the cells using
ViaFect Transfection Reagent
(
E4982
,
Promega
, Madison, WI, USA) according to the recommended protocol.
CRISPR-Cas9
knockout
screen in vivo The
(38)
The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster
iScience
November 18, 2022
Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik Hedenström, Björn Bohman, Marie Bengtsson, Paul G. Becher,
et al.
Belgium), sodium chloride (#1064041000, Merck, Darmstadt, Germany),
ViaFect™ Transfection Reagent
(#
E4981
,
Promega
, Walldorf, Germany), D-luciferin (
beetle
) monosodium salt (#E464X, Promega, Walldorf, Germany),
(39)
Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
October 28, 2022
Peng JY, Cai DK, Zeng RL, Zhang CY, Li GC, Chen SF, Yuan XQ, Peng L.
cells that had been cultured in a 6‐well plate with 3 µL of
ViaFect Transfection Reagent
(
Promega
,
E4982
). After
transfection
for 48 h, G418 (0.7 mg mL−1 for HepG2 cell line, 1 mg mL−1 for LM3 cell line) was
(40)
A synthetic transcription platform for programmable gene expression in mammalian cells
Nature Communications
October 18, 2022
William C. W. Chen, Leonid Gaidukov, Yong Lai, Ming-Ru Wu, Jicong Cao, Michael J. Gutbrod, Gigi C. G. Choi, Rachel P. Utomo, Ying-Chou Chen, Liliana Wroblewska,
et al.
USA) containing 10% FBS and 1% P/S.
Transfections
were carried out with
ViaFect transfection reagent
(
E4982
,
Promega
, Madison, WI, USA). Briefly, cells were washed once with PBS, trypsinized, and re-suspended
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Reviews
Write a Review
(1)
8/27/2023
High Efficiency Transfection Reagent From Promega
Samantha Chin
I used ViaFect as my transfection reagent for HEK293T to generate lentivirus. I had a very smooth experience with using ViaFect and achieved high-quality lentivirus generation. The added convenience ...
Read Review
(1)
6/17/2019
Easy-to-Use Transfection Reagent with Good Results from Promega
Wei Yang Sit
Highly recommended transfection reagent for those who intend to carry out transfection.
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ViaFect Transfection Reagent from Promega
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